cas9 fusion protein dd cas9 Search Results


cas9 protein  (New England Biolabs)
96
New England Biolabs cas9 protein
Cas9 Protein, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cas9 protein/product/New England Biolabs
Average 96 stars, based on 1 article reviews
cas9 protein - by Bioz Stars, 2026-02
96/100 stars
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cas9 protein  (TaKaRa)
95
TaKaRa cas9 protein
Cas9 Protein, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cas9 protein/product/TaKaRa
Average 95 stars, based on 1 article reviews
cas9 protein - by Bioz Stars, 2026-02
95/100 stars
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crispr/cas-9 genome editing  (Philips Healthcare)
90
Philips Healthcare crispr/cas-9 genome editing
Crispr/Cas 9 Genome Editing, supplied by Philips Healthcare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/crispr/cas-9 genome editing/product/Philips Healthcare
Average 90 stars, based on 1 article reviews
crispr/cas-9 genome editing - by Bioz Stars, 2026-02
90/100 stars
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px458 paxx crispr/cas9:gfp plasmid  (Thermo Fisher)
90
Thermo Fisher px458 paxx crispr/cas9:gfp plasmid
Px458 Paxx Crispr/Cas9:Gfp Plasmid, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/px458 paxx crispr/cas9:gfp plasmid/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
px458 paxx crispr/cas9:gfp plasmid - by Bioz Stars, 2026-02
90/100 stars
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cleaved caspase 9  (Abcam)
96
Abcam cleaved caspase 9
(A) The cell viability of AGS and AGS/CDDP detected by MTT ( P < 0.0001); (B) Apoptosis rate measured by Annexin V-FITC/PI assay ( P < 0.0001); (C) cleaved <t>PARP,</t> <t>cleaved</t> <t>caspase-9,</t> and cleaved caspase-3 displayed by western blot ( P = 0.0055, 0.0011, 0.0018); (D) MFAP2 detected by western blot ( P = 0.0023).
Cleaved Caspase 9, supplied by Abcam, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cleaved caspase 9/product/Abcam
Average 96 stars, based on 1 article reviews
cleaved caspase 9 - by Bioz Stars, 2026-02
96/100 stars
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recombinant cas9  (New England Biolabs)
98
New England Biolabs recombinant cas9
(A) The cell viability of AGS and AGS/CDDP detected by MTT ( P < 0.0001); (B) Apoptosis rate measured by Annexin V-FITC/PI assay ( P < 0.0001); (C) cleaved <t>PARP,</t> <t>cleaved</t> <t>caspase-9,</t> and cleaved caspase-3 displayed by western blot ( P = 0.0055, 0.0011, 0.0018); (D) MFAP2 detected by western blot ( P = 0.0023).
Recombinant Cas9, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant cas9/product/New England Biolabs
Average 98 stars, based on 1 article reviews
recombinant cas9 - by Bioz Stars, 2026-02
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pspcas9 bb 2a gfp cas9 expression plasmid  (Addgene inc)
96
Addgene inc pspcas9 bb 2a gfp cas9 expression plasmid
(A) The cell viability of AGS and AGS/CDDP detected by MTT ( P < 0.0001); (B) Apoptosis rate measured by Annexin V-FITC/PI assay ( P < 0.0001); (C) cleaved <t>PARP,</t> <t>cleaved</t> <t>caspase-9,</t> and cleaved caspase-3 displayed by western blot ( P = 0.0055, 0.0011, 0.0018); (D) MFAP2 detected by western blot ( P = 0.0023).
Pspcas9 Bb 2a Gfp Cas9 Expression Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pspcas9 bb 2a gfp cas9 expression plasmid/product/Addgene inc
Average 96 stars, based on 1 article reviews
pspcas9 bb 2a gfp cas9 expression plasmid - by Bioz Stars, 2026-02
96/100 stars
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human codon optimized streptococcus pyogenes cas9 nuclease  (Addgene inc)
93
Addgene inc human codon optimized streptococcus pyogenes cas9 nuclease
(A) The cell viability of AGS and AGS/CDDP detected by MTT ( P < 0.0001); (B) Apoptosis rate measured by Annexin V-FITC/PI assay ( P < 0.0001); (C) cleaved <t>PARP,</t> <t>cleaved</t> <t>caspase-9,</t> and cleaved caspase-3 displayed by western blot ( P = 0.0055, 0.0011, 0.0018); (D) MFAP2 detected by western blot ( P = 0.0023).
Human Codon Optimized Streptococcus Pyogenes Cas9 Nuclease, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human codon optimized streptococcus pyogenes cas9 nuclease/product/Addgene inc
Average 93 stars, based on 1 article reviews
human codon optimized streptococcus pyogenes cas9 nuclease - by Bioz Stars, 2026-02
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cleaved cas9 protein  (GE Healthcare)
97
GE Healthcare cleaved cas9 protein
(A) The cell viability of AGS and AGS/CDDP detected by MTT ( P < 0.0001); (B) Apoptosis rate measured by Annexin V-FITC/PI assay ( P < 0.0001); (C) cleaved <t>PARP,</t> <t>cleaved</t> <t>caspase-9,</t> and cleaved caspase-3 displayed by western blot ( P = 0.0055, 0.0011, 0.0018); (D) MFAP2 detected by western blot ( P = 0.0023).
Cleaved Cas9 Protein, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cleaved cas9 protein/product/GE Healthcare
Average 97 stars, based on 1 article reviews
cleaved cas9 protein - by Bioz Stars, 2026-02
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recombinant cas9  (Danaher Inc)
86
Danaher Inc recombinant cas9
( A ) Graphical representation of the wild type and mutated alleles and the screening strategy. Red bars show the position of the <t>Cas9</t> cut sites. ( B ) Results of the PCR genotyping showing wild type, heterozygous mutant, and knockout homozygous mutant.
Recombinant Cas9, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant cas9/product/Danaher Inc
Average 86 stars, based on 1 article reviews
recombinant cas9 - by Bioz Stars, 2026-02
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recombinant proteins cas9 protein  (Danaher Inc)
86
Danaher Inc recombinant proteins cas9 protein
( A ) Graphical representation of the wild type and mutated alleles and the screening strategy. Red bars show the position of the <t>Cas9</t> cut sites. ( B ) Results of the PCR genotyping showing wild type, heterozygous mutant, and knockout homozygous mutant.
Recombinant Proteins Cas9 Protein, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant proteins cas9 protein/product/Danaher Inc
Average 86 stars, based on 1 article reviews
recombinant proteins cas9 protein - by Bioz Stars, 2026-02
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Image Search Results


(A) The cell viability of AGS and AGS/CDDP detected by MTT ( P < 0.0001); (B) Apoptosis rate measured by Annexin V-FITC/PI assay ( P < 0.0001); (C) cleaved PARP, cleaved caspase-9, and cleaved caspase-3 displayed by western blot ( P = 0.0055, 0.0011, 0.0018); (D) MFAP2 detected by western blot ( P = 0.0023).

Journal: PeerJ

Article Title: MFAP2 enhances cisplatin resistance in gastric cancer cells by regulating autophagy

doi: 10.7717/peerj.15441

Figure Lengend Snippet: (A) The cell viability of AGS and AGS/CDDP detected by MTT ( P < 0.0001); (B) Apoptosis rate measured by Annexin V-FITC/PI assay ( P < 0.0001); (C) cleaved PARP, cleaved caspase-9, and cleaved caspase-3 displayed by western blot ( P = 0.0055, 0.0011, 0.0018); (D) MFAP2 detected by western blot ( P = 0.0023).

Article Snippet: Then the membrane was blocked with 5% skim milk and incubated with the primary antibody MFAP2 (A10230, 1:2000; ABclone, Wuhan, China), β -actin (ab8227, 1:10000; Abcam, Cambridge, UK), LC3II (ab192890, 1:2000; Abcam), cleaved-caspase-3 (ab32042, 1:500; Abcam), cleaved-PARP (ab32064, 1:1000; Abcam), cleaved-caspase-9 (ab32539, 1:1000; Abcam), Beclin 1 (ab207612, 1:1000; Abcam), ATG5 (ab108327, 1:1000; Abcam) and P62 (ab56416, 1:1000; Abcam) at 4 °C overnight and the second antibody (ab7090 or ab47827; Abcam), respectively.

Techniques: Western Blot

(A) The expression of MFAP2 (compared with AGS, P = 0.0113; compared with AGS/CDDP, P = 0.0136); (B) the cell viability at different concentrations of CDDP (IC50); (C) cells were cultured at 3 µg/mL CDDP for 24 h, and Annexin V-FITC/PI staining assay was applied to observe apoptosis rate (compared with AGS, P = 0.0375; compared with AGS/CDDP, P = 0.0098); (D) cells were cultured at 3 µg/mL CDDP for 48 h, and the content of pro-apoptotic proteins (cleaved PARP, cleaved caspase-3, and cleaved caspase-9) was detected (compared with AGS, P = 0.0009, 0.0025, 0.0089; compared with AGS/CDDP, P = 0.0023, 0.035, 0.0003).

Journal: PeerJ

Article Title: MFAP2 enhances cisplatin resistance in gastric cancer cells by regulating autophagy

doi: 10.7717/peerj.15441

Figure Lengend Snippet: (A) The expression of MFAP2 (compared with AGS, P = 0.0113; compared with AGS/CDDP, P = 0.0136); (B) the cell viability at different concentrations of CDDP (IC50); (C) cells were cultured at 3 µg/mL CDDP for 24 h, and Annexin V-FITC/PI staining assay was applied to observe apoptosis rate (compared with AGS, P = 0.0375; compared with AGS/CDDP, P = 0.0098); (D) cells were cultured at 3 µg/mL CDDP for 48 h, and the content of pro-apoptotic proteins (cleaved PARP, cleaved caspase-3, and cleaved caspase-9) was detected (compared with AGS, P = 0.0009, 0.0025, 0.0089; compared with AGS/CDDP, P = 0.0023, 0.035, 0.0003).

Article Snippet: Then the membrane was blocked with 5% skim milk and incubated with the primary antibody MFAP2 (A10230, 1:2000; ABclone, Wuhan, China), β -actin (ab8227, 1:10000; Abcam, Cambridge, UK), LC3II (ab192890, 1:2000; Abcam), cleaved-caspase-3 (ab32042, 1:500; Abcam), cleaved-PARP (ab32064, 1:1000; Abcam), cleaved-caspase-9 (ab32539, 1:1000; Abcam), Beclin 1 (ab207612, 1:1000; Abcam), ATG5 (ab108327, 1:1000; Abcam) and P62 (ab56416, 1:1000; Abcam) at 4 °C overnight and the second antibody (ab7090 or ab47827; Abcam), respectively.

Techniques: Expressing, Cell Culture, Staining

(A) The expression of MFAP2 (compared with AGS, P = 0.0047; compared with AGS/CDDP, P = 0.0036); (B) the protein content of p62, Beclin-1, ATG5 and LC3 I/II (compared with AGS, P = 0.0001, 0.00002, 0.0005, 0.00004; compared with AGS/CDDP, P = 0.011, 0.007, 0.0442, 0.011; compared with AGS/CDDP+siMFAP2, P = 0.0021, 0.0003, 0.0023, 0.0011); (C) the cell viability at different concentrations of CDDP (IC50); (D) the cell apoptosis after treatment with 3 µg/ml CDDP (compared with AGS, P = 0.0003; compared to AGS/CDDP, P = 0.0005; compared to AGS/CDDP+siMFAP2, P = 0.0227); (E) the protein content of cleaved PARP, cleaved caspase-3, and cleaved caspase-9 in the cells treated with 3 µg/ml CDDP (compared with AGS, P < 0.0001; compared to AGS/CDDP, P = 0.0002, 0.0001, 0.0031; compared with AGS/CDDP+siMFAP2, P = 0.0081, 0.012, 0.029).

Journal: PeerJ

Article Title: MFAP2 enhances cisplatin resistance in gastric cancer cells by regulating autophagy

doi: 10.7717/peerj.15441

Figure Lengend Snippet: (A) The expression of MFAP2 (compared with AGS, P = 0.0047; compared with AGS/CDDP, P = 0.0036); (B) the protein content of p62, Beclin-1, ATG5 and LC3 I/II (compared with AGS, P = 0.0001, 0.00002, 0.0005, 0.00004; compared with AGS/CDDP, P = 0.011, 0.007, 0.0442, 0.011; compared with AGS/CDDP+siMFAP2, P = 0.0021, 0.0003, 0.0023, 0.0011); (C) the cell viability at different concentrations of CDDP (IC50); (D) the cell apoptosis after treatment with 3 µg/ml CDDP (compared with AGS, P = 0.0003; compared to AGS/CDDP, P = 0.0005; compared to AGS/CDDP+siMFAP2, P = 0.0227); (E) the protein content of cleaved PARP, cleaved caspase-3, and cleaved caspase-9 in the cells treated with 3 µg/ml CDDP (compared with AGS, P < 0.0001; compared to AGS/CDDP, P = 0.0002, 0.0001, 0.0031; compared with AGS/CDDP+siMFAP2, P = 0.0081, 0.012, 0.029).

Article Snippet: Then the membrane was blocked with 5% skim milk and incubated with the primary antibody MFAP2 (A10230, 1:2000; ABclone, Wuhan, China), β -actin (ab8227, 1:10000; Abcam, Cambridge, UK), LC3II (ab192890, 1:2000; Abcam), cleaved-caspase-3 (ab32042, 1:500; Abcam), cleaved-PARP (ab32064, 1:1000; Abcam), cleaved-caspase-9 (ab32539, 1:1000; Abcam), Beclin 1 (ab207612, 1:1000; Abcam), ATG5 (ab108327, 1:1000; Abcam) and P62 (ab56416, 1:1000; Abcam) at 4 °C overnight and the second antibody (ab7090 or ab47827; Abcam), respectively.

Techniques: Expressing

( A ) Graphical representation of the wild type and mutated alleles and the screening strategy. Red bars show the position of the Cas9 cut sites. ( B ) Results of the PCR genotyping showing wild type, heterozygous mutant, and knockout homozygous mutant.

Journal: bioRxiv

Article Title: A positive feedback loop between germ cells and gonads induces and maintains cnidarian sexual reproduction

doi: 10.1101/2024.05.16.594501

Figure Lengend Snippet: ( A ) Graphical representation of the wild type and mutated alleles and the screening strategy. Red bars show the position of the Cas9 cut sites. ( B ) Results of the PCR genotyping showing wild type, heterozygous mutant, and knockout homozygous mutant.

Article Snippet: All sgRNAs were incubated together (500 ng/µl) with recombinant Cas9 (1 μg/μl; IDT, Cat. #1074181) for fifteen minutes prior to injection as described in .

Techniques: Mutagenesis, Knock-Out